Facebook PixelEngineered endosymbiosis: what would be some amazing collaborations?
Brainstorming session

Engineered endosymbiosis: what would be some amazing collaborations?

Engineered endosymbiosis: what would be some amazing collaborations?

Image credit: National Institutes of Health (NIH)

By Darko Savic on Oct 14, 2020

[1] https://en.wikipedia.org/wiki/Symbiogenesis

Creative contributions

Deinococcus radiodurans as safe-keeper of the host cell's DNA

[1] Minton KW. DNA repair in the extremely radioresistant bacterium Deinococcus radiodurans. Mol Microbiol. 1994 Jul;13(1):9-15. doi: 10.1111/j.1365-2958.1994.tb00397.x. PMID: 7984097.

by Darko Savic on Oct 14, 2020

Endosymbiosis engineering for containing anti-oxidant producing bacterial species

[1] Schieber M., Chandel N.S. ROS function in redox signaling and oxidative stress. Curr. Biol. 2014;24:R453–R462. doi: 10.1016/j.cub.2014.03.034

[2] Luo D., Fang B. Structural identification of ginseng polysaccharides and testing of their antioxidant activities. Carbohydr. Polym. 2008;72:376–381. doi: 10.1016/j.carbpol.2007.09.006.

[3] Lin, X., Xia, Y., Wang, G., Yang, Y., Xiong, Z., Lv, F., Zhou, W., & Ai, L. (2018). Lactic Acid Bacteria With Antioxidant Activities Alleviating Oxidized Oil Induced Hepatic Injury in Mice. Frontiers in Microbiology, 9, 1–17. https://doi.org/10.3389/fmicb.2018.02684

by Subash Chapagain on Oct 15, 2020

Mimic endosymbiosis: models and example

[1] Engineering yeast endosymbionts as a step toward the evolution of mitochondria Angad P. Mehta, Lubica Supekova, Jian-Hua Chen, Kersi Pestonjamasp, PaulWebster, Yeonjin Ko, Scott C. Henderson, Gerry McDermott, FrantisekSupek, Peter G. Schultz

[2] Ludington WB, Ja WW (2020) Drosophila as a model for the gut microbiome. PLoS Pathog 16(4): e1008398. https://doi.org/10.1371/journal.ppat.1008398

[3] Nigro G, Hanson M, Fevre C, Lecuit M, Sansonetti PJ. Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions. Methods Mol Biol. 2019;1576:183-194. doi: 10.1007/7651_2016_12. PMID: 27628134.

[4] Chowdhury, S., Castro, S., Coker, C. et al. Programmable bacteria induce durable tumor regression and systemic antitumor immunity. Nat Med 25, 1057–1063 (2019). https://doi.org/10.1038/s41591-019-0498-z

by Antonio Carusillo on Oct 17, 2020

Subash Chapagain 9 days ago
Elegantly framed concept.

While engineering endosymbiosis, not just horizontal gene transfer, immunogenicity can raise some problems (if we are looking to using non-native microorganisms). How would this approach address the potential risks of hyper-immune reactions?

Coming to the second case of using engineered E.coli against cancer cells, as far as I know, the receptor CD47 is not specific to tumours but it is needed for angiogenesis in normal tissues also (the only difference being overexpression in cancer cells). How does the engineered system (E.Coli) discriminate between the normal and cancer cells? Is it based on the CD47 marker only? Or are their other determinants which influence the recruitment of these E.coli cells against tumor?

Hence, the next stage in this pursuit would be to determine the specificity and efficacy of targeting and tagging non-normal cells.
Antonio Carusillo 9 days ago
Subash Chapagain 1- Since the microrganism will be engineered - something easier and easier to do - I think that we may identify the motifis used by our immune system to recognise them and eliminate them or change them in a way they won't be detected. We may even - that's why I have suggested it - look at our microbioma and see how our microbioma avoids to be attacked by our system ( what do they express and what not ) 2- As you well pointed out we will need more studies to better control the specificity of such microrganisms, in a way that they are directed only towards the target. Maybe by rendering them dependent ( auxotrophic ) on components present in a tumor environment only, so taht they can act only where the tumor is and only until the tumor is there. But this is a pure guess.

Make the symbiont's DNA incomplete without the host cell's DNA

by Dragan Otasevic on Oct 14, 2020

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Dragan Otasevic 12 days ago
Take a look at these photosynthetic animals https://www.youtube.com/watch?v=AcX2n1rC4W4