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What methods can be used to control in vivo cellular reprogramming?

Image credit: Ocampo et al. 2016 (https://www.sciencedirect.com/science/article/pii/S0092867416316646)

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Jamila
Jamila Aug 04, 2020

[1]Ma, Xiaojie, Linghao Kong, and Saiyong Zhu. "Reprogramming cell fates by small molecules." Protein & cell 8.5 (2017): 328-348.

[2]Ocampo, Alejandro, et al. "In vivo amelioration of age-associated hallmarks by partial reprogramming." Cell 167.7 (2016): 1719-1733.

[3]Tamanini, S., G. P. Comi, and S. Corti. "In vivo transient and partial cell reprogramming to pluripotency as a therapeutic tool for neurodegenerative diseases." Molecular neurobiology 55.8 (2018): 6850-6862.

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Use of artificial transcription factors

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Shubhankar Kulkarni
Shubhankar Kulkarni Aug 05, 2020
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Shubhankar Kulkarni
Shubhankar Kulkarni2 months ago
Here is a paper that has identified and listed the transcription factors that can be used for reprogramming (https://www.nature.com/articles/s41587-020-0742-6#citeas). They have presented the Human TFome, a library containing 1,564 TF genes and 1,732 TF splice isoforms. They discovered 290 TFs (including 241 novel ones), that induce differentiation in 4 days. The cell-autonomous approach enables parallel programming of hPSCs into multiple cell types simultaneously. These can be used to construct similar synthetic transcription factors that may avoid the problems with the natural ones.

Regenerative Medicine: somatic cell reprogramming with drugs cocktail .

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Facu Garcia Barberá
Facu Garcia Barberá Sep 19, 2020
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Jamila
Jamila 4 months ago
In a previous study, bioinformatics and deep learning methods were applied to the datasets available on the LINCS website (http://www.lincsproject.org/). This helped to determine the gene(s) and pathway(s) patterns involved in drugs like metformin and rapamycin. The researchers were able to screen drug matches with hundreds of compounds using this method. (https://pubmed.ncbi.nlm.nih.gov/29165314/) Perhaps this method could be used to identify reprogramming agents similar to the ones we already know about.

Calcium: age-related bioelement in thrombosis process.

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Facu Garcia Barberá
Facu Garcia Barberá Sep 19, 2020

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