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Why are embryonic stem cell lines genetically different from the person they are taken from?

Why are embryonic stem cell lines genetically different from the person they are taken from?

Image credit: https://deakincomsci2016.wordpress.com/2016/04/17/organ-regeneration-and-tissue-repair/

By Shubhankar Kulkarni on Aug 13, 2020

[1] Cascalho M, Platt JL. The Future of Organ Replacement: Needs, Potential Applications, and Obstacles to Application. Transplant Proc [Internet]. 2006 Mar;38(2):362–4. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0041134505015290

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Combining existing technologies to overcome problems with organ regeneration

by Apoorva Kulkarni on Aug 13, 2020

Shubhankar Kulkarni 2 months ago

Blastocyst complementation: Although this does not answer the question of why are the embryonic stem cell lines different, this provides a solution for xenogenic organ generation for transplantation. In a recent study, researchers have successfully used blastocyst complementation to generate a functional kidney. Mouse embryonic stem cells were harvested. They were injected into pseudo-pregnant rat uteri along with Sall1 mutant rat blastocysts. The Sall1 mutants do not have functional kidneys. The complemented blastocysts matured into normal fetuses. More than two-thirds of the resulting rat progeny contained a pair of kidneys derived from the mouse stem cells. The kidney was structurally intact, retained the characteristics of the stem cell donor mouse, and about 50% of them were functional. They could be potentially be used for transplantation. However, extending this strategy to humans might create a new set of challenges. Reference: Goto T, Hara H, Sanbo M, Masaki H, Sato H, Yamaguchi T, et al. Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats. Nat Commun [Internet]. 2019 Dec 5;10(1):451. Available from: http://www.nature.com/articles/s41467-019-08394-9...

Time-accumulated DNA errors and imperfect corrections

by Darko Savic on Aug 13, 2020

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