An experiment to determine what it would take to achieve limb regeneration in humans
Image credit: Yang Yang, Bei Liu, et al.
Please leave the feedback on this idea
Is it original or innovative?
Is it feasible?
Is it targeting an unsolved problem?
Is it concisely described?
- axolotl blastocyst (host) + human pluripotent stem cells
- mouse blastocyst (host) + human pluripotent stem cells
- Amputate a limb in both adult chimeras and look for human cells at the amputation spot (limb bud blastema).
- The mouse-human chimera serves as a control in this experiment. This is because neither species has regeneration capabilities in adulthood.
- The axolotl-human chimera would hopefully retain the regeneration capability and grow a new limb.
- Do a side by side comparison of gene expression and other "omics", especially epigenomics.
- The result hints at what conditions need to be present for human limb regeneration.
James D, Noggle SA, Swigut T, Brivanlou AH: Contribution of human embryonic stem cells to mouse blastocysts. Dev Biol 2006;295:90-102.
Freedman B, S: Hopes and Difficulties for Blastocyst Complementation. Nephron 2018;138:42-47. doi: 10.1159/000480370
Mascetti VL, Pedersen RA: Human-mouse chimerism validates human stem cell pluripotency. Cell Stem Cell 2016;18:67-72.