An experiment to determine what it would take to achieve limb regeneration in humans
Image credit: Yang Yang, Bei Liu, et al.
- axolotl blastocyst (host) + human pluripotent stem cells
- mouse blastocyst (host) + human pluripotent stem cells
- Amputate a limb in both adult chimeras and look for human cells at the amputation spot (limb bud blastema).
- The mouse-human chimera serves as a control in this experiment. This is because neither species has regeneration capabilities in adulthood.
- The axolotl-human chimera would hopefully retain the regeneration capability and grow a new limb.
- Do a side by side comparison of gene expression and other "omics", especially epigenomics.
- The result hints at what conditions need to be present for human limb regeneration.
James D, Noggle SA, Swigut T, Brivanlou AH: Contribution of human embryonic stem cells to mouse blastocysts. Dev Biol 2006;295:90-102.
Freedman B, S: Hopes and Difficulties for Blastocyst Complementation. Nephron 2018;138:42-47. doi: 10.1159/000480370
Mascetti VL, Pedersen RA: Human-mouse chimerism validates human stem cell pluripotency. Cell Stem Cell 2016;18:67-72.