How justifiable is 16S rDNA sequences for taxonomic identification of bacteria?
Image credit: https://www.peoplespharmacy.com/articles/does-your-microbiota-change-when-you-take-nexium
Please leave the feedback on this challenge
Is the problem still unsolved?
Is it concisely described?
Bounty for the best solution
Provide a bounty for the best solution
Bounties attract serious brainpower to the challenge.
Only 16S analysis is not reliable if the similarity is more than 97%
- Sequencing the housekeeping genes: Sometimes, 16S sequences may match perfectly but the housekeeping gene sequences do not. They are then used to identify species.
- Whole-genome sequencing: With sequencing techniques readily available and the reduced cost of sequencing, the entire genomes are sequenced, so that the researchers can play around looking at different genes specific only to that genus for further detailed identification.
- Chemotaxonomy: The polar lipid content and the fatty acid content are some of the criteria used to identify a species.
- Biochemical analysis: To detect the presence of certain enzymes, such as catalases, oxidases, ureases, gelatinases, etc., that are produced by the bacteria.