Currently one of the most important problems of innovative genome engineering is effective in vivo intracellular delivery of the tissues of interest. All of the common vectors have their limitations. However, now can create an artificial vector that could alloy the best natures of the conventional vectors by the technology of so-called de novo virus synthesis.
Adeno-associated Virus AAV:
insert size limit of 4.5kb, requirement of adenovirus or herpes virus for AAV replication, low transduction efficiency, difficult generation of high titers
possible cytopathic effects
possible toxicities, risk of recombination
transient gene expression, an immune response to viral proteins, insert size limit of 7,5 kb
transfect only proliferating cells, a concern of insertional mutagenesis, a difficult target of viral infection
potential insertional mutagenesis