Facebook PixelEradicate oral bacteria with bacteriophage viruses and replace them with genetically modified, protective strains

Eradicate oral bacteria with bacteriophage viruses and replace them with genetically modified, protective strains

Eradicate oral bacteria with bacteriophage viruses and replace them with genetically modified, protective strains

Image credit: Kukski

By Darko Savic on Aug 02, 2020

[1] Szafrański, Szymon P et al. “The use of bacteriophages to biocontrol oral biofilms.” Journal of biotechnology vol. 250 (2017): 29-44. doi:10.1016/j.jbiotec.2017.01.002

[2] Loesche WJ. Microbiology of Dental Decay and Periodontal Disease. In: Medical Microbiology. 4th ed. University of Texas Medical Branch at Galveston, Galveston (TX); 1996.

[3] Hillman, Jeffrey D., et al. "Safety assessment of ProBiora3, a probiotic mouthwash: subchronic toxicity study in rats." International journal of toxicology, 28.5, 2009, pp. 357-367.

[4] Hillman, J. D., S. S. Socransky, and Myra Shivers. "The relationships between streptococcal species and periodontopathic bacteria in human dental plaque." Archives of Oral Biology, 30.11-12, 1985, pp. 791-795.

[5] Hillman, J. D., et al. "A spontaneous lactate dehydrogenase deficient mutant of Streptococcus rattus for use as a probiotic in the prevention of dental caries." Journal of applied microbiology, 107.5, 2009, pp. 1551-1558.

[6] Socransky, S. S., et al. "Associations between microbial species in subgingival plaque samples." Oral microbiology and immunology, 3.1, 1988, pp. 1-7.

Darko Savic 2 months ago

Are there any populations (tribes, etc) known to have extremely low incidence of oral diseases?...

Shubhankar Kulkarni 2 months ago

I think dental caries arise predominantly due to lack of sufficient immune response rather than the presence or absence of specific microorganisms. Maintaining immunity may, therefore, be the best way to go. We may extrapolate this issue to all pathogens like acne- or other disease-causing bacteria residing on the skin and we need to carry out the above-mentioned process for all the human organs typically associated with pathogens. This seems like a time- and resource-consuming process. On the other hand, maintaining immunity is a one-shot solution to all such problems....

Darko Savic 2 months ago

Do you propose that with a properly functioning immune system dental caries and other oral infections, would not be a problem?...

Darko Savic 2 months ago

In some individuals and with some bacteria the immune system will respond with an innate-only response. Others will need to invoke the inflammatory response, and yet others will require the adaptive immune response (cellular, humoral, or both). As the biofilm increases in complexity and variety so does the immune response. So "properly functioning" is elusive. ...

Jamila Ahmed 2 months ago

Many bacteria including S. mutans are challenged by bacteriophages in the environment. As a result, bacteria have developed a defence mechanism against bacteriophages called the CRISPR-Cas system. Several studies have shown that most s. mutans strains possess the CRISPR-Cas system and thus can avoid an attack by specific bacteriophages like phage M102. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7316486/) However, some phages have also developed mechanisms to avoid the bacterial CRISPR-Cas system by producing anti-CRISPRs. (https://science.sciencemag.org/content/369/6499/54) Perhaps there are bacteriophages that naturally possess specific anti-CRISPRs that can reduce caries-causing bacteria, and these specific phages could be selected for the mouthwash or anti-CRISPR proteins could be added to the mouthwash separately and this may enhance the effectiveness of the bacteriophages against caries-causing bacteria. ...

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